Boston Electronics Corp.

Paper 13324-25 NADH, FAD and TRP metabolic FLIM: tuneable excitation from 400 nm – 1300 nm for 1p vs. 2p imaging of the same sample area (Invited Paper) 27 January 2025 • 10:45 AM - 11:00 AM PST | Moscone South, Room 301 (Level 3) FLIM experiments typically rely on basic assumptions for interpretation. First, the absorption and emission spectra may not translate from pure samples to ‘real’ biological samples. Second, a chosen excitation may excite a plethora of fluorophores other than the target. It is, therefore, crucial for comparative studies to have precise tuneable control of the excitation wavelength. Third, the absorption spectra for 1-p and 2-p excitation of most fluorophores are not exactly a factor 2 apart, a common assumption. We present a tunable (400 nm – 1300 nm) femtosecond FLIM microscopy system to compare 1-p and 2-p results from the same sample area. Presenter Julius Heitz Becker & Hickl GmbH (Germany)

Paper 13324-15 Metabolic NADH FLIM: precise, turnkey quantitative FLIM, FLIM-FRET and more in zen for the Zeiss LSM980 (Invited Paper) 26 January 2025 • 3:50 PM - 4:10 PM PST | Moscone South, Room 301 (Level 3) We present NADH metabolic imaging FLIM results and an intuitive turnkey FLIM solution for the Zeiss LSM980 – controlled in the Zen software. Based on the full precision and efficiency of Becker&Hickl GmbH TCSPC technology, data is recorded, sent through the SPCimage NG analysis pipeline, and results are returned to Zen for comparison. Advantages include: direct visualisation of and access to all fit parameters, lifetime analysis and multi-parameter segmentation of 2D/3D data sets, and unsurpassed lifetime resolution with a system IRF down to ~20ps @203 fs bins to resolve even the smallest lifetime differences. Available for new and existing Zeiss LSM980. Presenter Lukas Braun Becker & Hickl GmbH (Germany)

- Eight Fully Parallel Timing Channels on a Single PC Board - Expandable to 32 Parallel Channels - GHz-Bandwidth Input Discriminators - 1 ps Time-Channel Width - 500 MHz Peak Count Rate Please see https://www.becker-hickl.com/products/spc-qc-008-tcspc-module/

- Ultra-fast TCSPC/FLIM module - Down to 200 femtoseconds time channel width - <1.2 ps timing jitter - GHz-bandwidth constant-fraction discriminators - Ultimate time resolution for B&H fast HPM-series hybrid detectors - Ideal for superconducting SSPD detectors Please see https://www.becker-hickl.com/products/spc-180n-series/

- Short Pulse Width - Excellent Timing Stability - Excellent Power Stability - No Warm-Up Time - Fast On / Off Modulation - Wide Selection of Wavelengths - Small Size - Circularised Free-Space Output - Single-Mode Fibre Output - Compatible with all bh TCSPC / FLIM Systems For details please see https://www.becker-hickl.com/products/category/lasers/

- Four Wavelengths from One Single-Mode Fibre - Short Pulse Width - Excellent Timing Stability - Excellent Power Stability - No Warm-Up Time - Fast Multiplexing Function - Compatible with all bh TCSPC / FLIM Systems For details please see https://www.becker-hickl.com/products/laser-hub/

- Quantitative FLIM - Molecular Imaging - Metabolic Imaging - FRET Imaging - Entry Level to High-End Applications For details please see https://www.becker-hickl.com/products/category/laser-scanning-microscopes/

• Legendary for Precision and Fit Stability • Combination of Time-Domain and Phasor Analysis • MLE Fit • Fast GPU Processing • Automatic IRF Generation • Global Analysis Procedure • FLIM Images within Seconds • Superior Accuracy of Multi-Exponential Decay Analysis • Compatible with all BH FLIM and TCSPC Systems For details please see https://www.becker-hickl.com/products/spcimage

• FLIM Functions Embedded in Zeiss ZEN Software • Entry Level to High-End Molecular Imaging • Ultra-High Time Resolution • Ultra-High Sensitivity • Advanced Multi-Dimensional Recording Capabilities • Legendary SPCImage NG FLIM Data Analysis For details please see https://www.becker-hickl.com/products/flim-upgrades-for-zeiss-lsm-family-microscopes/

• FLIM Functions Embedded in Zeiss ZEN Software • Entry Level to High-End Molecular Imaging • Ultra-High Time Resolution • Ultra-High Sensitivity • Advanced Multi-Dimensional Recording Capabilities • Legendary SPCImage NG FLIM Data Analysis For details please see https://www.becker-hickl.com/products/flim-upgrades-for-zeiss-lsm-family-microscopes/

- Eight Fully Parallel Timing Channels on a Single PC Board - Expandable to 32 Parallel Channels - GHz-Bandwidth Input Discriminators - 1 ps Time-Channel Width - 500 MHz Peak Count Rate Please see https://www.becker-hickl.com/products/spc-qc-008-tcspc-module/

- Quantitative FLIM - Molecular Imaging - Metabolic Imaging - FRET Imaging - Entry Level to High-End Applications For details please see https://www.becker-hickl.com/products/category/laser-scanning-microscopes/

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